Paper describing ReSOLVIt published in FSI:Genetics

We modify our full in silico DNA pipeline to focus on resolving signal-to-noise within the single-copy regime.

The paper describes system design and ways in which the system can be implemented into forensic DNA validation and optimization.

Paper Highlights

•An in silico DNA system is parameterized with the laboratories own experimental data, resulting in predictions of optimal laboratory settings.
•Noise and allele peak height distributions from a single copy of DNA are used to assess signal-to-noise resolution.
•Optimal signal detection thresholds, or analytical thresholds, for casework are obtained, if necessary.
•We demonstrate that metrics of signal quality for simulated and experimental data are consistent.
•This is a systematic method for evaluating EPG quality and is a critical step towards standardizing the post-PCR process.